zikv puerto rico strain prvabc59  (ATCC)

Journal: Journal of Virology

Article Title: Zika virus infection in neonatal mice disrupts connexin 43 and induces cardiac inflammation, implicating viral myocarditis in neonatal pathogenesis

doi: 10.1128/jvi.00871-25

Figure Lengend Snippet: ZIKV replication, heart enlargement, and spleen alterations in neonatal mice. One-day-old C57BL/6 mice were injected intraperitoneally with the ZIKV strain PRVABC59 (10⁶ TCID₅₀/mouse) or PBS as a control, and serum and tissue samples were collected at 0, 1, 6, and 11 dpi. ( A ) ZIKV RNA levels in serum and heart tissue were quantified by real-time RT-PCR using ZIKV-specific primers ( n = 3/group). ( B ) Representative images of whole hearts from ZIKV-injected and PBS-treated mice at 6 and 11 dpi. Scale bars, 2 mm. ( C and D ) Absolute heart weight ( C ) and heart-to-body weight ratio (HW/BW) ( D ) of ZIKV-infected and PBS-treated mice at 1, 6, and 11 dpi ( n = 3–10 per group). Error bars indicate SEM. (E–G) Effects of ZIKV infection on spleen morphology and weight. ( E ) Representative images of whole spleens from ZIKV-infected and PBS-treated mice at 6 and 11 dpi. Scale bars, 2 mm. ( F, G ) Spleen-to-body weight ratio (SW/BW) ( F ) and absolute spleen weight ( G ) in ZIKV-infected and PBS-treated mice at 1, 6, and 11 dpi ( n = 3–10 per group). Statistical significance: ns, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: The clinical isolate of Asian lineage ZIKV strain PRVABC59 (GenBank accession number KU501215 , Puerto Rico, 2015) ( ) and ZIKV strain MR766 were purchased from ATCC and used in this study.

Techniques: Injection, Control, Quantitative RT-PCR, Infection

Journal: Journal of Virology

Article Title: Zika virus infection in neonatal mice disrupts connexin 43 and induces cardiac inflammation, implicating viral myocarditis in neonatal pathogenesis

doi: 10.1128/jvi.00871-25

Figure Lengend Snippet: Electrocardiogram (EKG) abnormalities in neonatal mice following ZIKV infection. ( A ) One-day-old C57BL/6 mice were injected intraperitoneally with ZIKV strain PRVABC59 and were recorded for approximately 10 min using a direct-writing oscillograph. Representative 450 ms segments of high-quality EKG tracings were shown for ZIKV-infected (left) and PBS-treated (right) mice. ( B ) Representative EKG tracings of neonatal mice at 11 dpi, comparing ZIKV-infected and PBS-treated controls. ( C ) QRS duration in ZIKV-infected vs PBS-treated mice ( n = 3–7 per group). *** P < 0.001.

Article Snippet: The clinical isolate of Asian lineage ZIKV strain PRVABC59 (GenBank accession number KU501215 , Puerto Rico, 2015) ( ) and ZIKV strain MR766 were purchased from ATCC and used in this study.

Techniques: Infection, Injection

Journal: Journal of Virology

Article Title: Zika virus infection in neonatal mice disrupts connexin 43 and induces cardiac inflammation, implicating viral myocarditis in neonatal pathogenesis

doi: 10.1128/jvi.00871-25

Figure Lengend Snippet: Expression levels of cytokines and chemokines in neonatal mice following ZIKV infection. One-day-old C57BL/6 mice were injected intraperitoneally with PRVABC59 (10 6 TCID 50 /mouse) or PBS. ( A ) Protein array analysis and quantification. Levels of 40 different cytokines and chemokines were measured in serum samples collected at 11 dpi using Mouse Cytokine Array ( n = 3 per group). Left panel: a representative image from triplicate samples is shown, with elevated cytokines and chemokines highlighted by squares and labeled below. The most elevated cytokines, CCL2 and CXCL10, were indicated by an asterisk. Right panel: Data from triplicates were quantified by Image J Software, normalized to positive and negative controls, and presented as a bar graph. ( B ) Cytokine/chemokine quantification assay. Serum and heart tissue samples were collected at 6 and 11 dpi ( n = 3-5 per group), and the expression levels of 32 cytokines and chemokines were measured using Luminex technology and analyzed by xPONENT Software. Upregulated cytokines or chemokines are underlined in red. Statistical significance: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: The clinical isolate of Asian lineage ZIKV strain PRVABC59 (GenBank accession number KU501215 , Puerto Rico, 2015) ( ) and ZIKV strain MR766 were purchased from ATCC and used in this study.

Techniques: Expressing, Infection, Injection, Protein Array, Labeling, Software, Luminex

Journal: Journal of Virology

Article Title: Zika virus infection in neonatal mice disrupts connexin 43 and induces cardiac inflammation, implicating viral myocarditis in neonatal pathogenesis

doi: 10.1128/jvi.00871-25

Figure Lengend Snippet: Levels of cardiac muscle enzyme in blood and heart tissues. Three-day-old C57BL/6 mice were injected intraperitoneally with MR766 and PRVABC59 (10 4 TCID50/mouse) or PBS. Serum and heart tissue samples were collected at 6 and 12 dpi ( n = 8 per group) for cardiac muscle enzyme detection. ( A, B, F ) The concentrations of cTnT, cTnI, and CK-MB in the serum and heart tissue samples were measured by a UMIC Wan200+ analyzer according to the manufacturer’s protocols. (C through E) Levels of LDH, α-HBDH, and CK were measured by a UMIC BC2000 analyzer according to the manufacturer’s protocols. Statistical significance: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: The clinical isolate of Asian lineage ZIKV strain PRVABC59 (GenBank accession number KU501215 , Puerto Rico, 2015) ( ) and ZIKV strain MR766 were purchased from ATCC and used in this study.

Techniques: Injection

Journal: Journal of Virology

Article Title: Zika virus infection in neonatal mice disrupts connexin 43 and induces cardiac inflammation, implicating viral myocarditis in neonatal pathogenesis

doi: 10.1128/jvi.00871-25

Figure Lengend Snippet: Pathological changes in the heart tissues of ZIKV-infected neonatal mice. Heart tissues were collected at 11 dpi from 1-day-old C57BL/6 mice injected intraperitoneally with ZIKV strain PRVABC59 (10 6 TCID 50 /mouse) or PBS. ( A ) Immunohistochemical (IHC) staining of Cx43 protein. Representative images of heart tissue cross-sections stained for Cx43 (brown). Scale bars: 100 µm on the left side and 20 µm on the right side. ( B ) Masson’s trichrome staining and IHC analysis of ZIKV protein. Upper panel: Masson trichrome staining highlights cardiac fibrosis (blue). Lower panel: IHC detection of ZIKV protein using anti-NS3 monoclonal antibody (7A9), with positive signals appearing brown. Scale bars: 100 µm (upper) and 20 µm (lower). ( C ) IHC staining of cleaved caspase-3 in the heart tissue. Representative images of heart tissue sections stained with anti-cleaved caspase-3 antibody. Positive apoptotic signals appear brown. Scale bars: 100 µm (left) and 20 µm (right).

Article Snippet: The clinical isolate of Asian lineage ZIKV strain PRVABC59 (GenBank accession number KU501215 , Puerto Rico, 2015) ( ) and ZIKV strain MR766 were purchased from ATCC and used in this study.

Techniques: Infection, Injection, Immunohistochemical staining, Immunohistochemistry, Staining

Journal: Journal of Virology

Article Title: Zika virus infection in neonatal mice disrupts connexin 43 and induces cardiac inflammation, implicating viral myocarditis in neonatal pathogenesis

doi: 10.1128/jvi.00871-25

Figure Lengend Snippet: ZIKV infection reduces Cx43 protein levels in neonatal heart tissue and isolated cardiomyocytes. ( A ) Western blotting analysis of the heart tissues. Heart tissues were collected at 11 dpi from the 1-day-old C57BL/6 mice injected intraperitoneally with ZIKV strain PRVABC59 (10 6 PFU/mouse) or PBS ( n = 3/group). Homogenized heart tissues were subjected to western blot assays for Cx43, Tubulin, and ZIKV NS3 detection. ( B ) Western blotting analysis of the cardiomyocytes. Cardiomyocytes were isolated from the hearts of six 1-day-old mice and infected with ZIKV (MOI = 1) for 24 h. MG132 (5 or 10 µM), a ubiquitin-proteasome process inhibitor, was added to the cells 6 h before harvesting the cells. Whole-cell lysates were analyzed by western blot using anti-Cx43, anti-ZIKV NS3, and anti-tubulin antibodies. ( C ) RT-qPCR analysis of Cx43 mRNA levels. Relative Cx43 mRNA levels were measured in mock- or ZIKV-infected cardiomyocytes at 12, 24, and 48 hpi, normalized to GAPDH expression.

Article Snippet: The clinical isolate of Asian lineage ZIKV strain PRVABC59 (GenBank accession number KU501215 , Puerto Rico, 2015) ( ) and ZIKV strain MR766 were purchased from ATCC and used in this study.

Techniques: Infection, Isolation, Western Blot, Injection, Ubiquitin Proteomics, Quantitative RT-PCR, Expressing